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Biochemistry/Metabolism

Application notes

TI0853

LC-MS: Thyroid hormone

TI0849

LC-MS: Orexin A and B

TI0848

LC-MS: Orexin A and B

TI0847

LC-MS: Orexin A and B

TI0846

LC-MS: Kynurenine metabolism pathway

TI0837

LC-MS: SAH and SAM

TI0834

LC-MS: Acidic compound (mobile phase pH)

TI0831

LC-MS: Insulin (Mobile phase pH)

TI0830

LC-MS: Amyloid beta-peptide [Human,1-40] (Mobile phase pH)

TI0829

LC-MS: Amyloid beta-peptide [Human,1-40] (alkali conditions)

TI0822

Lipid A (Salmonella minnesota Re595)

TI0821

Recombinant human serum albumin (rHSA)

TI0820

Recombinant and biosynthetic human serum albumin (HSA)

TI0819

Recombinant human serum albumin (rHSA)

TI0818

LC-MS: retro-dipeptide isomers

TI0817

LC-MS, Biochemistry/Metabolism, Amino Acids and Peptides

TI0816

LC-MS: choline metabolites and related compounds

TI0815

LC-MS: isoproterenol

TI0814

LC-MS: carnitine related compounds

TI0813

LC-MS: Amino acids in human serum (HClO4)

TI0812

LC-MS: Amino acids in human serum (SSA)

TI0811

LC-MS: dipeptides

TI0807

LC-MS: Epinephrine and metanephrine

TI0806

LC-MS: Synthetic polyamines

TI0805

LC-MS: Biogenic polyamines

TI0803

LC-MS/MS: Acetylcholine and d4-acetylcholine

TI0802

LC-MS: Neuroblastoma related compounds

TI0801

LC-MS: Neurotransmitters (2)

TI0733

The Unison UK-Amino column successfully separates adenosine and inosine, which are very similar in structure.

TI0732

The Scherzo SM-C18 and Unison UK-Amino columns successfully separate the small, polar ascorbic acid related compounds in normal phase mode.

TI0731

The Scherzo SS-C18 column provides better retention of Shikimic Acid than a standard C18 column.

TI0727

Cadenza CW-C18, Unison UK-C18, and Unison UK-C8 are all able to resolve the very polar and charged Acetyl CoA and CoA, differing only in an acetyl group.

TI0720

The Scherzo SM-C18 and Scherzo SS-C18 column resolve very polar carboxylic acids that normally co-elute on a standard C18 column (Unison UK-C18).

TI0719

The Scherzo SS-C18 column retains Prostaglandins the most, followed by Scherzo SM-C18 and Scherzo SW-C18.

TI0718

Under neutral pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

TI0717

Under acidic pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

TI0716

On the Unison UK-C18 column, tetrahydrofuran gives better resolution of Prostaglandins than acetonitrile and acetone.

TI0715

Acetonitrile and methanol have opposite retention properties in the separation of prostaglandins on the Unison UK-C18 column.

TI0710

The Scherzo SM-C18 column successfully resolves nucleotide triphosphates of similar structure.

TI0704

The Scherzo SM-C18 column can separate these two molecules, which would normally coelute on a standard C18 column.

TI0701

The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of these small molecules as the standard Unison UK-C18. However, the Scherzo SS-C18 shows the best resolution between these three compounds.

TI0698

The Scherzo SS-C18 column retains and separates glutamic acid and GABA better than a conventional C18 column

TI0694

Small, polar molecules like neurotransmitters are difficult to retain on a conventional C18 column like Unison UK-C18. Scherzo SS-C18 can retain these compounds much better than a standard Unison UK-C18 column, as well as resolve the peaks better. Conditions compatible with LC-MS

TI0691

The highly charged phosphate groups make separation of AMP, ADP, and ATP difficult on a conventional C18 column. Scherzo SW-C18 is able to elute these compounds effectively, in both ammonium acetate and ammonium phosphate buffers.

TI0684

Cytochrome c

TI0683

Serotonin related compounds

TI0682

Histamine related compounds

TI0681

Neurotransmitters

TI0671

Zwitterionic compounds

TI0670

Zwitterionic compounds

TI0669

Catecholamines

TI0652

Exploring different C18 stationary phases for metabolites profiling

TI0649

Simulatneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids

TI0648

Simulatneous HPLC/LT-ELSD Analysis of Water Soluble and Fat Soluble Vitamins

TI0642

Scherzo is able to separate mandelic acid and dopamine, which coelute on a conventional C18 column (Unison UK-C18). This is possible because the ionic ligands recognize the small structural differences between the two compounds.

TI0641

Catecholamine metabolites

TI0634

The effect of gradient slope and column length for thyroglobulin separation

TI0630

The effect of gradient slope for insulin separation

TI0629

The effect of gradient slope for angiotensin II serparation

TI0612

Unsaturated Chondro-Disaccharides

TI0608

LC-MS(MRM) Application for Monosaccharides

TI0607

Organic acids (Anion exchange mode)

TI0601

Urea and related compounds

TI0599

Coenzymes (Nicotinamide adenine dinucleotides)

TI0598

Coenzymes (Nicotinamide adenine dinucleotides)

TI0588

Cyclic mononucleotides (cAMP, cGMP)

TI0548

Organic acids (2)

TI0542

Organic Acids

TI0539

Purine bases

TI0536

Methimazole and potential metabolites

TI0530

Levulinic Acid, Lactic Acid and methyl Esters

TI0529

Adenosine Phosphates

TI0524

Anserine and Related Compounds

TI0522

Mononucleotides

TI0521

Glutathiones

TI0520

Sodium Chloride

TI0519

Mevalonolactone and Mevalonic Acid

TI0518

Organic Acids

TI0517

Estradiol and Metabolites

TI0512

Identification of sphingomyelin molecular species with LC-MS/MS

TI0493

LC-Q-TOF-MS application for high-throughput non-targeted metabolomics with a hybrid ODS column

TI0491

LC-MS/MS Application for Sphingolipids (Sphinganine-1-PO4)

TI0490

LC-MS/MS Application for Sphingolipids (Sphingosine-1-PO4)

TI0489

LC-MS/MS Application for Sphingolipids (Sphingosine)

TI0488

LC-MS/MS Application for Sphingolipids (Sphinganine)

TI0487

LC-MS/MS Application for Sphingolipids (Ceramide)

TI0477

LC-MS Application for Phospholipids

TI0466

Creatinine and Purine Metabolites

TI0464

LC-MS/MS Application for 25-Hydroxy Vitamin D3

TI0462

Unsaturated Chondro-Disaccharides

TI0454

Cyclic Mononucleotides

TI0449

Coenzymes (Nicotinamide adenine dinucleotides)

TI0448

Adenosine Phosphates (AMP, ADP, ATP)

TI0447

Sugar Phosphates

TI0446

Sugar Phosphates

TI0429

Alpha-keto acids

TI0421

PQQ: pyrroloquinoline quinone (Normal Phase)

TI0417

Xanthines and Metabolites

TI0416

Xanthines and Metabolites in Bovine Serum

TI0415

Xanthines and Metabolites

TI0409

5-Fluorouracil and Related Compounds

TI0406

Nucleosides and Bases

TI0397

LC-MS/MS Application - Steroid Hormones and Metabolites

TI0393

The Unison UK-Amino column shows excellent recognition of both the oxidized and reduced forms of glutathione.

TI0379

Nicotine derivatives and metabolites can be separated by both reversed phase and normal phase chromatography. Reversed phase gives better run time.

TI0377

Unison UK-Amino is able to separate amino acid-like molecules with good resolution.

TI0376

Cadenza HS-C18 successfully separates out small molecules like Estradiol and metabolites from bovine serum. The sample chromatogram looks almost identical to the standard chromatogram.

TI0375

Many Cadenza and Unison columns achieve excellent resolution with beta-Estradiol 3,17-disulfate and 17beta-estradiol despite their similar structures.

TI0371

Unison UK-C18 successfully separates methylated arginines of similar structure. Compounds are pre-column derivatized to enable fluroescence detection.

TI0370

Unison UK-C18 successfully separates nitroNE, nitroE, and nitroDA. Detection is via post-column derivatization.

TI0362

Unison UK-Amino column separates catecholamines of similar structure using normal phase/anion exchange mode.

TI0360

Unison UK-Amino column separates out taurine in a drink mixture.

TI0357

Mandelic acid and related compounds

TI0345

Hippuric acids

TI0339

Creatine and metabolites

TI0338

LC-MS of marajuana and its metabolite in blood

TI0333

Sialic acid and uronic acids

TI0257

Causative Substances of Urolithiasis

TI0247

Separation properties of basic compounds on CL-C18

TI0246

Separation properties for acidic compounds on CL-C18

TI0245

Retention behavior of polar compounds

TI0241

 

TI0227

LC-ELSD of taurine and water soluble vitamins

TI0226

Separation of creatine and related polar metabolites with 100% aqueous mobile phase, using the UK-C18, which excels in the separation of highly polar compounds.

TI0195

Analysis of coenzyme Q10, a dietary supplement used for its antioxidant action, with various columns.

TI0193

 

TI0192

Separation of UDP-sugars, intermediates in sugar chain synthesis, the molecular structure of which differs only in the sugar group.

TI0191

Reverse phase analysis of the highly polar compound betaine, which is quite difficult to retain in normal phase analysis.

TI0190

Separation of short chain cable fatty acids without modifying the functional group.

TI0184

Normal phase separation of the neurotransmitter GABA.

TI0181

This analysis of xanthine oxydase related compounds includes separation of its substrate, product and inhibitor.

TI0180

Analysis of ATP, ADP and AMP in aqueous normal phase using a silica column.

TI0179

Reverse phase analysis of purine nucleotide metabolites, including uric acid and allantoin.

TI0178

Normal phase analysis of purine nucleotide metabolites, including uric acid and allantoin, using a silica column.

TI0174

Analysis of the highly polar catecholamines. The ion pair reagent, not included, is separated by the mobile phase.

TI0170

The polar compound L-Carnitine, analyzed in both normal and reverse phase modes.

TI0151

Caproic acid and its methylvaleric acid isomers are separated with high resolution using our 500mm long column.

TI0132

Reverse phase analysis of dye-labeled urea, a highly polar compound.

TI0131

Simultaneous analysis of Citric Acid Cycle compounds using our phenyl column.

TI0130

Simultaneous analysis of Citric Acid Cycle compounds using our UK-C18 column.

TI0129

Simultaneous analysis of Ornithine Cycle comounds using our UK-C18, which has affinity for highly polar compounds.

TI0127

High-speed separations with Unison UK-C8 150mm column compared with a 5um 250mm column popular on the market.

TI0115

Retention properties of polar compounds

TI0053

Analysis of creatine in chewing gum

TI0047

Separation of inosinic acid, a flavor enhancer.

TI0012

Separation of hippuric acid, a metabolite of toluene and xylene. Cadenza can recognize positional isomers of methyl hippuric acid as well.

SP0028

An Evaluation of a Multi-Mode Stationary Phase for HPLC Separations Using a Multi-Component Test Mix

SP0027

Advantage of a New Generation of Evaporative Light-Scattering Detectors: Universality, Higher Sensitivities and Cost-Effectiveness for Multi-Element Analyses in Liquid Chromatography - An Application Review

SP0025

High-throughput non-targeted metabolic profiling with hybrid stationary phase LC column coupled to Q-TOF-MS in cancer research

SP0015

Ultra High Sensitivity Analysis of Testosterone

JA0006

Spin Tip Solid-Phase Extraction And HILIC-MS-MS For Quantitative Determination Of Methamphetamine And Amphetamine In Human Plasma