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For analysis of polar solutes in high aqueous conditions

TARGA HPLC columns and cartridges have become a popular choice for LC-MS applications, especially ones involving problematic basic compounds. The inert surface chemistry characteristics and relatively high surface area provide a unique selectivity without peak tailing even for basic samples.

More importantly, problematic solutes such as basic peptides can be analyzed without TFA in the mobile phase which greatly enhances detection limits. Buffers as weak as 0.01% acetic or formic acid are routinely used for peptide mapping and peptide analysis.

TARGA HPLC columns work well with 100% aqueous mobile phases and equilibrate quickly when running gradients beginning with 100% water.

High Speed Microbore Analysis

  • High stability and low back pressure (150bar) even at 6 times the optimum flow rate
  • Basic drugs don't tail on TARGA columns when using only a mild formic acid buffer that is ideal for LC/MS applications
  • The TARGA column shows unique selectivity, performs well, and equilibrates quickly in 100% aqueous conditions.

Column stability and efficiency are primary issues for researchers developing fast HPLC methods with low detection limits. An example of a very high throughput plasma drug analysis illustrates several performance features of Higgins Analytical's TARGA microbore columns.


200µL of plasma is precipitated with MeCN, evaporated to dryness, then dissolved in 10µL of 10% MeOH/ water solution. The 2.5µL injection on a TARGA C18 100x1mm column is equivalent to 50µL of plasma. A 0 - 95% MeCN vs 0.1% aqueous formic acid gradient is run at 300µL/min over 5min, held for 1min, then returned to 0% MeCN by 6.1min. The analysis is repeated every 8 minutes.

TARGA graph

Download a Higgins Analytcal Catalogue Here