Scherzo SW-C18 has a novel
structure, with a small amount of strong ionic ligand combined with
octadecyl silane. This structure provides better elution of strong
ionic compounds. siRNA (small interfering RNA), around 21 base
paired double stranded RNA, is getting lots of attention for next
generation medicines. But it is very polar and contains a lot of
phosphoric acid groups and is therefore difficult to analyse by
Reverse Phase. Figure7a shows multiple peaks of commercial siRNAs
which may include different structural compounds. SW-C18 recognizes
the different number of nucleic bases (hydrophobic interaction) and
phosphoric acids (ionic interaction). SW-C18 can separate not only
siRNA, but also ATP or oligo-nucleotides without adding an
ion-pairing reagent to the eluent.
- Polar and non-polar retention in Reversed Phase conditions
- Multi-level retention without ion-pairing reagents
- Compatible with LC-MS
- Most unique selectivity of any HPLC column on the market
today
- Wide range of method development possibilities
- Can simultaneous optimize organic, buffer and pH gradients
- Anion Exchange + Cation Exchange + Reversed Phase + Normal
Phase
- Low amount of strong ionic ligands, effective for strong ionic
compounds
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SCHERZO SW C18
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Synthetic rna
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APPLICATIONS